AZ-NO3 - Thirty Day Nitrate Remover
All Natural Oxidation/Reduction Enzyme Catalyst
(Absolute Zero - Nitrates)
On this page, below, is the condensed and reformated text from the AZ-NO3 Product Information Pamphlet distributed to Retailers as a hand-out to their customers.
An internal link is also provided, at the bottom of the page, to the unabridged full AZ-NO3 Dealers Information Report booklet in pdf format, for those so inclined to learn more about our marvelous product.
AZ-NO3 works entirely by aerobic Cellular Respiration on the target Nitrate molecule, which is then removed by the Protein Skimmer. The oxidoreductases necessary for these processes are the chemical mechanism of enzyme action.
In the simplest of explanations, AZ-NO3 can be considered an essential food, required by the active enzyme maltase that functions as a cellular respirator. The manufacturing process renders this product as an undesirable food to certain detrimental enzymes (amylase and invertase) that could uncontrollably proliferate in the aquarium.
Our product utilizes a specific enzyme in the maltase group to activate the oxidoreductases necessary to reduce nitrates by cellular respiration in the aquarium. The product itself does not contain this enzyme, but can be thought of more like a seed, a seed contains the germ (plant) and the food for the plant to grow. The mature plant then becomes the basic food product utilized.
The activating ingredient in our product is Cozymase which function in the oxidation of proteins and of many other compounds important in the intermediary metabolism and the resulting active enzyme Maltase in the aquarium. The Massecuite is food for both the Cozymase to develop and the resultant Maltase to thrive.
AZ-NO3 is packaged in a convenient twin cap *measuring and dispensing bottle, the product is Non-Toxic, thus the bottle may be reused around the aquaria for dispensing many other liquid products as well.
To utilize the measuring feature of the container, the user simply removes the cap from the dispensing side of the container and squeezes the bottle until the liquid level in the dispenser is at the desired dosage level. To dispense, the bottle is inverted over the sump, aquarium or doser.
* Reader Please Note: This expensive measuring and dispensing bottle was replaced with a much lower cost recyclable squeeze bottle with a drip dispensing cap. Thus making it easier to follow the drip method dosing directions. Our product price was also lowered considerably (over 25%) when the new lower priced bottles came into usage and the saving passed along to the consumer.
The proper denitrifying dosage of the product is 1/4 ounce, daily (after the initial drip start method is completed), for each 50 to 60 gallons of water in the aquarium system, until nitrates are undetectable. Overdosing, (up to double the amount) is preferred to underdosing and is not harmful to livestock. Larger aquariums will require a larger dose, but nitrates are normally reduced to zero within the same time period.
Many aquarists inadvertently introduce nitrates on a continuing basis, through water changes, food, additives, etc. therefore requiring that they maintain the enzyme activity at a low maintenance dosage to hold nitrates in check.
Initially the maintenance dosage should be 1/2 of the denitrifying dosage, steadily decreasing the amount of AZ-NO3 used until nitrates can be maintained at an undetectable level.
The intent is to slowly wean the aquarium off the AZ-NO3 product entirely, allowing the natural denitrification properties of the aquariums live rock and/or plenum to come into full functioning. The maintenance dosage can be rescheduled for bi-weekly or even weekly additions as the live rock or substrate develops to it's full potential.
An alternative start-up method is to use 1/2 the recommended amount or less (see Safe-Start method on bottle label) for the first 7 days, then increasing to the proper dosage during the denitrifying period, then slowly decreasing the maintenance dosage until the aquarium is weaned from the product. This alternative method extends the usefulness of the product and also reduces the undesirable bloom effect mentioned under SHORT TERM EFFECTS on the next page.
AZ-NO3 provides many other benefits besides nitrate reduction. Upon addition of the product to the aquarium, you will notice that the Protein Skimmer becomes more efficient and remains that way for approximately 30 days after the final use of the AZ-NO3 product. In addition, as the enzymes become active, all sediment and pre-filters in the aquarium remove larger amounts of undesirable organics, thus requiring more frequent filter cleaning.
If an enzyme activating product has never been used in the aquarium, it is most likely that it will experience a short bacterial bloom, extending from day 3 thru day 5 of the program, indicated as a whitish haze in the water. The alternative start-up methods is recommended, to reduce or eliminate the amount of cloudiness as the beneficial bacteria become active. If ionic imbalance, or undesirable enzymes already exist in the aquarium, a very prominent white haze may result. This clouding is harmless and will dissipate as the enzymes become active or the imbalance is corrected.
You may not notice any clouding or haziness in the water if the aquarium is in perfect ionic balance or you have previously used an enzyme activating product. Some foods and additives provide certain enzyme activating compounds that also keep the aquarium from clouding.
The AZ-NO3 product was extensively tested in rigorous laboratory testing and scrutinized by over 85 home marine and reef aquarists (when it was first released nearly two decades ago now). The only negative findings reported that certain animals showed a temporary adverse reaction to the product during the start-up phase of the program. These include: Sea Mat, Mushroom and Green Star Polyps; Leather Corals (all species); Bubble and Elegance Corals; and a few Feather Duster Worms. However, in all cases, the animals reopened fully and became more luxuriant.
In an effort to explain how the AZ-NO3 product functions, one must understand the enzyme, the catalyst, how they function and what reactions are produced. Enzymes are substances that cause and direct the numerous chemical reactions that occur in living organisms. Many chemical reactions will not proceed except in the presence of minute amounts of certain substances called catalysts. Enzymes are the catalysts of living cells.
In order to understand the full significance of this definition, it is necessary to grasp that the living organism may be regarded as a system of chemical reactions, beautifully integrated and co-ordinated, of extraordinary complexity, but amenable to experimental investigation and to some measure of comprehension. Most chemical changes that occur in living cells are caused and controlled by enzymes, that exhibit the physical and chemical properties of protein molecules.
Enzymes show a remarkable degree of specificity for the substances on which they act. A particular enzyme catalyzes only one kind of reaction and acts only on one kind of substance. This high degree of specificity is one of the characteristics that sharply differentiates enzymes from other catalysts and allows the AZ-NO3 product to single out and remove nitrates from the aquarium.